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evaluation of isotope 32p method to mark culex pipiens (diptera: culicidae) in a laboratory
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نویسنده
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zhang chongxing ,shi guihong ,zhao yuqiang ,yan dongmei ,li huaiju ,liu hongmei ,wiwatanaratanabutr itsanun ,gong maoqing
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منبع
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journal of arthropod-borne diseases - 2016 - دوره : 10 - شماره : 2 - صفحه:211 -221
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چکیده
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background: the aim of the current study was to develop a marking technique as an internal marker to mark post blood meal mosquitoes by using stable phosphate isotope 32p and determine the optimal concentration of it.
methods: an isotonic physiological saline solution, containing different concentration of radioactive isotope 32p-labeled disodium phosphate (na2h32po4) was injected into rabbits via the jugular vein in the laboratory. emerged cx. pipiens were marked after feeding on rabbit. at the same time, the labeled conditions of emerged cx. pipiens were also measured by placing feces of no. 6 rabbit into containers with mosquito larvae and pupae inside.
results: according to the label condition of cx. pipiens after taking blood and the effect of different dosage na2h32po4 on rabbit health, the optimal concentration of radioactive isotope was determined, that is, 0.1211 mci/kg. by placing feces of no. 6 rabbit into containers with mosquito larvae and pupae inside, the emerged mosquitoes were also labeled. therefore, feeding mosquitoes on the animal injected with radioactive na2h32po4 was more practical for detecting and tracing mosquitoes.
conclusion: the method was less time-consuming, more sensitive and safer. this marking method will facilitate post-bloodmeal studies of mosquitoes and other blood-sucking insects.
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کلیدواژه
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radioactive isotope ,mark ,culex pipiens ,rabbit
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آدرس
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shandong institute of parasitic diseases, shandong academy of medical sciences, jining, shandong, people’s republic of china, China, shandong institute of parasitic diseases, shandong academy of medical sciences, jining, shandong, people’s republic of chinacollaborative innovation center for the origin and control of emerging infectious diseases, taishan medical university, taian, shandong 271000, china, China, shandong institute of parasitic diseases, shandong academy of medical sciences, jining, shandong, people’s republic of china, China, shandong institute of parasitic diseases, shandong academy of medical sciences, jining, shandong, people’s republic of china, China, shandong institute of parasitic diseases, shandong academy of medical sciences, jining, shandong, people’s republic of china, China, shandong institute of parasitic diseases, shandong academy of medical sciences, jining, shandong, people’s republic of china, China, department of plant production technology, faculty of agricultural technology, king mongkut's institute of technology ladkrabang, bangkok 10520, thailand, China, shandong institute of parasitic diseases, shandong academy of medical sciences, jining, shandong, people’s republic of china, China
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پست الکترونیکی
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maoqingg@yahoo.com
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Authors
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