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In molecular biology, real-time polymerase chain reaction, also called quantitative real time polymerase chain reaction (qpcr) is a laboratory technique based on the pcr, which is used to amplify and quantify a targeted dna molecule at the same time. for one or more specific sequences in a dna sample, real-time pcr enables both detection and quantification. the quantity can be either an absolute number of copies or a relative amount when normalized to additional normalizing endogenous controls. also, real-time pcr is combined with reverse transcription to quantify mrna and non-coding rna in cells or tissues.