In Vitro Propagation of Orchid Phalaenopsis Amabilis (L.) Blume Var. Jawa

A protocol was developed for high frequency in vitro multiplication of an ornamental orchid, phalaenopsis amabilis (l.) blume var. jawa, using plant growth regulators (pgrs). protocormlike bodies (plbs), as explants were cultured on murashige and skoog (ms) medium fortified with various concentrations of kinetin (kin) and indole-3-butyric acid (iba), either individually or in combination. a combination of 1.00 mg l^-1 kin + 1.00 mg l^-1 iba was found to be suitable for regeneration of most measured characteristics especially maximum plbs regeneration (30.40/plantlet), leaf number (5.93/plantlet), and root number (8.36/plantlet) from protocorm explants. the maximum number of plantlets (11.66) was calculated on ms medium supplemented with 1.00 mg l^-1 kin + 0.50 mg l^-1 iba, followed by 1.00 mg l^-1 kin + 1.00 mg l^-1 iba (10.33). plantlets were transplanted to pots filled with cocochips and sphagnum moss (70:30) for acclimatization and transferred to the greenhouse. upon ex vitro transfer, 90% of plants survived.