ارزیابی نتاج و معرفی ژنوتیپ های برتر نسل دوم حاصل از تلاقی طالبی ’سمسوری‘با رقم تجاری ’گالیا‘

ارقام بومی طالبی در ایران کیفیت و طعم مطلوبی دارند ولی به‌دلیل حساسیت به بیماری‌های قارچی و ویروسی و عملکرد پایین در مقایسه با ارقام هیبرید وارداتی به تدریج از چرخه تولید حذف می‌شوند. به‌منظور ترکیب صفات و تشکیل جمعیت اصلاحی، طالبی رقم ’سمسوری‘ با یک رقم هیبرید تجاری از گروه گالیا که در سال‌های اخیر به‌دلیل مقاومت به بیماری‌ها، قطر گوشت و شیرینی بالا بسیار مورد استقبال جالیزکاران قرار گرفته است، تلاقی داده شد. در این پژوهش تعداد 1000 عدد گیاه نسل دوم حاصل از تلاقی رقم ’سمسوری‘ با رقم ’کوری‘، به همراه بذرهای والدی و نسل اول آنها کشت شدند تا به روش شجره‌ای، بوته ها ضمن خودگشنی، در شرایط طبیعی مزرعه برای صفات زراعی، مقاومت به بیماری‌ها و در آزمایشگاه برای صفات کمی و کیفی میوه مورد ارزیابی و گزینش قرار گیرند. نتایج نشان داد همه صفات کمی مورد ارزیابی، بجز ضخامت پوست میوه (0.661) و شاخص شکل میوه (0.531) دارای وراثت‌پذیری عمومی بالای 85 در‌صد بودند. میانگین همه صفات کمی به جز مواد جامد محلول (10.8) و ضخامت گوشت میوه (3.52) در جمعیت f1 نسبت به میانگین والدین کمتر بود. در جمعیت f2 برای تمام صفات کمی تفکیک متجاوز مثبت نسبت به والد برتر و تفکیک متجاوز منفی نسبت به والد با ارزش کمتر مشاهده شد. در جمعیت f2 نزدیک به 80 درصد ژنوتیپ ها کاملاً مشبک و 20 درصد دارای میزان شبکه کمتر و یا بدون شبکه روی پوست میوه بودند. این نتایج با نسبت های ژنتیکی 3 به 1 مندلی همخوانی داشت. همچنین 35 درصد ژنوتیپ های جمعیت f2 خط‌دار بودند و بیش از 80 درصد آنها رنگ پوست میوه متمایل به زرد و رنگ گوشت میوه سبز داشتند. با توجه به اینکه انتخاب براساس صفات با وراثت‌پذیری بالا در نسل های اولیه قابل اعتماد و موفقیت آمیزتر خواهد بود، بنابراین بهتر است گزینش ژنوتیپ های برتر در این جمعیت به‌ترتیب براساس صفات مهم اقتصادی، وزن میوه، مواد جامد محلول و زودرسی صورت گیرد.

evaluation of progeny and introduction of superior genotypes of f2 generation resulting from crossing of samsoori cantaloupe by galia commercial cultivar

introduction cantaloupes, are a group of cucumis melo cultivars, that have round, fragrant fruits with netted skin. our country, is one of the top five producers of cantaloupe, and melon in the world. its original origin, is still debated, but one of the important centers of its diversity, is iran. although, native cultivars, such as, saveh cantaloupe, samsoori, rish baba, and various types of tiles, are often of good quality, and taste, but they are sensitive to a variety of fungal and viral diseases and as a result, their yield is low. on the other hand, every year new cultivars and hybrids, are introduced by seed companies, which are welcomed by farmers, due to, their better agronomic characteristics, and resistance to a wider range of diseases. continuation of this process, in addition to severe genetic erosion of native cultivars, and populations, and even the complete elimination of some of them, will have consequences such as, dependence of production on foreign companies, annual departure of currency from the country, and non-exploitation of the country’s genetic resources. therefore, it seemed that by creating a diverse population resulting from the crossing of superior native cultivars with cultivars of commercially resistant hybrid to diseases, and then, successive selections, new cultivars, with desirable traits of both parents, could be achieved. thus, in this study, heritability, minimum and maximum mean values of parents, and f1 and f2 generations, aggressive segregation, and relative frequency of qualitative traits, in f2 generation, and selection of the best genotypes, in two dispersing ngf2 populations, were investigated. materials and methods parental cross, was performed in the spring of 2017, between samsoori cantaloupe cultivar (round, striped fruit, cream skin color, completely reticulate, green flesh, very early ripening fruit, poor transportability and durability, free pollinator and high homogeneity) as the selected native paternal parent, and commercial cultivar from gallia group, that called cory and as maternal parent (round fruit, completely reticulate, no striped skin, yellow to cream skin color, high transportability and durability, green and very sweet flesh, high resistance to various plant diseases and viral diseases) and produced by seminis company. the first-generation seeds, obtained from parental crosses, were planted in the greenhouse in the fall of 2017, and were self-sown. in the spring of 1397, 1000 seeds of f2 generation, along with parent seeds, and their first generation, were planted in a seedling tray, in the greenhouses of the faculty of agricultural technology (aborihan) of tehran university, and transplanted to the ground, in the rental research production farm, located, in filistan village-golzar sector-pakdasht. controlled pollination, (isolation of male flowers, and manual inoculation) was performed, for all second-generation plants. selection for subsequent generations, was done by pedigree method, all plants in f2 generation, were evaluated, and selected. morphological traits, such as, plant form and phenological traits, such as, day to fruit harvest, were evaluated, and recorded. plant health, was assessed against common and important fungal, viral and mites, under normal field conditions by scoring the severity of infections in four categories. fruit quality traits were measured based on nominal, sequential and interval scales and quantitative fruit traits were also measured. tables of minimum and maximum values, variances, general heritability, transgressive segregation, etc for quantitative traits, also relative frequency percentages and observed genetic ratios for quantitative traits, were calculated. results and discussion the results showed that all quantitative traits in the f2 population had a general heritability above 90%. the mean of all quantitative traits except soluble solids and fruit flesh thickness in f1 population was lower than the parental average. in f2 population, for all quantitative traits, positive transgressive segregation was observed, compared to, the superior parent, and negative transgressive segregation was observed, compared to, the less valuable parent. in the f2 population, nearly 80% of the genotypes, were completely reticulate, and 20% had less or no netting, on the fruit skin. these results were almost consistent with genetic ratios of 3.1. also, 35% of f2 population genotypes, were striped and more than 80% of them had yellowish skin color and green flesh color. due to, the fact that, selection based on traits with high heritability, will be more reliable, and successful in early generations, so, it is better, to select the best genotypes, in this population, based on fruit weight, soluble solids, and early maturity, respectively.  conclusionin the f2 population, positive transgressive segregation was evident for all quantitative traits, favoring the superior parent, while negative segregation was observed for the less valuable parent. traits exhibiting high heritability showcased a pronounced influence of genetic variance over environmental variance. consequently, selecting cultivars based on these traits in early generations is expected to yield more reliable and successful outcomes. therefore, it is better, to selection, the best genotypes in the early generations, in the f2 population, based on fruit weight, (with high genetic efficiency), soluble solids, day to fruit ripening, and with the pedigree management, of the populations, it is finally, possible to achieve lines, that have the desirable traits, of both parental cultivars. the resulting lines, can be used to produce, new hybrids, or provided, to the farmer, as a single cultivar.