اثر بسترهای کشت بر برخی صفات مورفولوژیکی و فیزیولوژیکی دو رقم ریحان در سیستم آکواپونیک

انواع مختلفی از تکنیک های کشت بدون خاک در تولید محصولات کشاورزی توسعه یافته اند از جمله سیستم های کشت هیدروپونیک و آکواپونیک. سیتم کشت آکواپونیک تلفیقی است از تولید آبزیان و هیدروپونیک که در آن از فضولات ماهی در تغذیه ی گیاهان استفاده می‌شود. هدف از انجام این تحقیق مقایسه‌ی برخی صفات مورفولوژیکی، فیزیولوژیکی و عملکرد دو رقم ریحان ’سبز‘ و ’بنفش‘ تحت تاثیر بسترهای مختلف در سیستم کشت آکواپونیک بود. این تحقیق براساس طرح آزمایشی فاکتوریل بر پایه طرح کاملاً تصادفی با سه تکرار در یک گلخانه ی تحقیقاتی در زنجان انجام شد. تیمارها شامل: ده بسترکشت کوکوپیت، پرلیت، ماسه، پوکه معدنی، و مخلوط50 درصد (کوکوپیت و پرلیت، کوکوپیت و پوکه معدنی، کوکوپیت و ماسه، پوکه معدنی و ماسه، پوکه معدنی و پرلیت، ماسه و پرلیت)، و دو رقم ریحان (’سبز‘ و ’بنفش‘) بود. پس از گذشت 4 ماه برخی صفات رشدی ریحان مورد ارزیابی قرار گرفت. براساس نتایج تجزیه واریانس برهم کنش متقابل رقم × بستر کشت بر صفات تعداد گره، وزن خشک اندام هوایی، وزن خشک ریشه، سطح برگ، تعداد برگ، وزن تر ریشه و ارتفاع گیاه معنی دار شد. اثر متقابل رقم × بستر بر صفات فیزیو فیتوشیمیایی شامل کلروفیل a، کلروفیل کل و آنتوسیانین دارای اثر معنی دار در سطح احتمال 1 درصد بود اما بر صفات کلروفیل b و کاروتنوئید اثر معنی داری را نشان نداد. در این تحقیق بیشترین مقدار صفات رشدی ارقام ریحان در بستر کشت ترکیبی پرلیت و کوکوپیت به دست آمد. رقم ریحان ’بنفش‘ در بسیاری از صفات از رشد بیشتری نسبت به رقم ریحان ’سبز‘ در سیستم کشت آکواپونیک برخوردار بود. به طور کلی بسترهای کشت حاوی پرلیت و کوکوپیت از رشد مطلوب تری نسبت به سایر بسترهای کشت برخوردار بودند و در بسترهای ماسه و پوکه معدنی ارقام ریحان رشد کمتری را نشان دادند.

effect of substrates on some morphological and physiological traits of two basil cultivars in aquaponic system

introductionthe aquaponic cultivation system, which is a combination of aquaculture and hydroponic systems, is among the novel cultivation systems. in an aquaponics system, it is possible to simultaneously produce aquatic animals and vegetables. one of the most important components of each soil-free system is the selection of a suitable substrate. this substrate makes water and nutrients available for the root system and also contributes to the proper establishment of the plant. it must also be non-toxic and should not impair plant growth. another point to consider in the selection of the substrate is its cost and availability, as the transport process will increase the costs of initial establishments in such systems. amon vegetables, basil is a special crop with a short growing season and high economic value, which is also highly suitable for an aquaponic system. the present study was carried out with the aim of comparing some morphological and physiological traits of ’purple’ and ’green’ basil cultivars in different substrates in the aquaponics system.materials and methods the present study was conducted as a factorial based on a completely randomized design with three replicates in a research greenhouse located in zanjan city. the experimental treatments included 10 substrates (cocopeat, perlite, sand, pumice, cocopeat + perlite (50:50), cocopeat + pumice (50:50), cocopeat + sand (50:50), pumice + sand (50:50), pumice + perlite (50:50) and sand + perlite (50:50)) and two basil cultivars (’green’ and pueple). the aquaponic system in this study comprised of a 300 l fish tank containing 100 common carps. the fish were nurtured twice a day with powdered fish feed containing protein, carbohydrates, lipids, vitamins, etc. the water in the fish tank was filtered and fish wastes were removed in a filtration tank before entering the aquaponics medium to be used by the plant root system. chemical properties of the fish water including ph, and nitrite (no2), nitrate (no3), and ammonium (nh4) concentrations were controlled using diagnostic kits on a weekly basis. at the flowering stage, various traits including plant height, dry and fresh weights of roots and shoots, number of nods, number of leaves, and leaf area were measured to investigate the growth of basil plants. also, physio-phytochemical traits including the contents of chlorophyll a, chlorophyll b, total chlorophyll, carotenoid and anthocyanin were measured. analysis of variance of the data was carried out using the sas software. also, the least significant difference method (lsd) was used for the comparison of means.results and discussionaccording to the results, the greatest plant height (60.57 cm), shoot fresh weight (90.57 g), root fresh weight (22.33 g), and leaf number (133.99) belonged to purple basil and the lowest value for these traits were observed in green basil cultivar. the results of the mean comparison for the morphological traits indicate the superiority of perlite + cocopeat compared with the other substrates. basil plants cultivated in sand substrate exhibited the lowest values for growth traits compared to other treatments. analysis of the interaction effect between substrate and cultivar revealed that the highest number of nodes (10.5), dry weight of shoots (25.17 g), dry weight of roots (4.67 g), and leaf area (17.13 cm2) were observed in the perlite + cocopeat substrate with purple basil. conversely, the lowest values for these traits (5.23, 12.93 g, 1.46 g, and 11.03 cm2, respectively) were recorded in the silt substrate with the green basil cultivar. the results related to physio-phytochemical traits showed that the highest content of chlorophyll a (1.57 mg g -1 fw) and total chlorophyll (2.1 mg g -1 fw) were observed in the perlite + cocopeat substrate and green basil. also, anthocyanin content was the highest (0.17 mg g -1 fw) when the purple cultivar was grown in the perlite + c cocopeat substrate. the lowest content of chlorophyll a and total chlorophyll were recorded in the sand substrate and purple cultivar with 0.96 and 1.17 mg g -1 fw, respectively. also, the lowest content of anthocyanin was related to the green basil cultivar with 0.058 mg g -1 fw. conclusionoverall, the ’purple’ cultivar of basil was superior to the ’green’ cultivar regarding most traits in the aquaponic system. also, substrates containing perlite and cocopeat led to better results compared with the other substrates, whereas the basil plants grown in sand and pumice had lower growth, relatively.