ارزیابی تنوع ژنتیکی برخی از ژنوتیپ‌های زرشک وحشی (.berberis spp) در استان‌های البرز و تهران با استفاده از نشانگرهای ریخت‌شناسی و میوه‌شناسی

زرشک از گیاهان بومی ایران و متحمل به تنش های محیطی بخصوص خشکی است. تنوع ژنتیکی 25 ژنوتیپ زرشک وحشی در دامنه های شمالی استان های تهران و البرز با استفاده از خصوصیات مورفولوژیکی مورد بررسی قرار گرفت. 32 صفت کمّی و کیفی مورفولوژیکی و باغبانی بر اساس دستورالعمل upov (tg 3.68) ارزیابی شدند که از این تعداد 11 صفت کمی قابل اندازه گیری شامل‌ طول و عرض برگ، طول و عرض میوه، طول دمگل، تعداد حبه در خوشه، طول خار، ویتامین ث، ta (titratable acidity) و tss (total soluble solid) مورد تجزیه و تحلیل قرار گرفت. برای تعیین فاصله ژنتیکی بین ژنوتیپ ها از روش‌های آماری چند متغیره شامل تجزیه به عامل ها، تجزیه خوشه ای و ضرایب همبستگی بین صفات استفاده شد. نتایج تجزیه واریانس اختلاف معنی‌داری را بین ژنوتیپ‌ها برای تمام صفات کمّی مورد ارزیابی نشان داد. تعداد حبه در خوشه با 45.55 ، طول خار با 28.67 و اسید قابل تیتراسیون با 26.58 درصد بیشترین دامنه تغییرات را داشتند. ژنوتیپ های وحشی زرشک با دارا بودن حداقل میزان 4.7 میلی گرم ویتامین ث در صد گرم میوه تازه (ژنوتیپ آسارا یک) و بیشترین مقدار ویتامین ث 10.57 (طالقان 4) و 9.63 (چالوس 8) و 9.4 (طالقان 8) در مقایسه با میوه های معتدله مانند سیب، انگور، گیلاس و هلو قابل توجه هستند. طبق تجزیه خوشه ای ژنوتیپ‌های زرشک وحشی به پنج گروه اصلی و ده زیر گروه و یک ژنوتیپ مستقل (چالوس 9) تقسیم شدند. در اکثر موارد ژنوتیپ‌ها با موقعیت اقلیمی یکسان رویشی در یک گروه قرار گرفتند. ژنوتیپ های گروه یک بالاترین طول و عرض میوه، طول و عرض برگ و طول خار را داشتند. در این میان ژنوتیپ های چالوس 8 و طالقان 8 با دارا بودن مقدار بالای ویتامین ث، در یک زیر گروه قرار گرفتند. ژنوتیپ های طالقان4، چالوس 8 و  طالقان 8 با بیشترین میزان ویتامین ث قابل اهمیت در برنامه‌های اصلاحی هستند. تنوع مشاهده شده در بین ژنوتیپ های زرشک وحشی زمینه مناسبی برای برنامه‌های اصلاحی و انتقال صفات مطلوب می باشد.

Investigation of Genetic Diversity of Some Wild Barberry Genotypes (Berberis spp.) in Alborz and Tehran Provinces Using Morpho-pomological Markers

Introduction Barberry is one of the native plants of Iran and tolerates environmental stresses, especially drought. This species can play a special role in the future development of horticulture in Iran. This species is drought tolerant and can be used in the development of rainfed gardens. The Barberry family contains 15 genera and 650 species, most of which are distributed in the temperate regions of the Northern Hemisphere. The most important genus in the barberry family is Berberis. This genus has 500 species, some of which, including Zalzalaki, Zarafshani, Khorasani, Rastkhoshe, common and Japanese barberry, exist in Iran (i.e., Berberis vulgaris, B. orthobotrys, B. crataegina, B. integerrima, and B. khorasanica, respectively). This plant is widely distributed in Iran. Various wild species of barberry are distributed in the southern and northern slopes of Alborz from Firoozkooh, Taleghaneh, Miyaneh to Amarlu of Gilan. This genus grows on large areas of the Zagros at an altitude of 1000 to 2500 meters above sea level. Iran is the largest producer of seedless barberry in the world with a cultivated area of ​​18341 hectares and production of 21181 tons of dried barberry. This species can play a special role in the future development of horticulture in the country and reduce the pressure on water resources, especially in rainfed horticulture.Materials and Methods In this study, 25 genotypes were evaluated from three regions: west of Alborz province (Taleghan region), north of Alborz province (Chalus road and Khuzenkola, Arangeh, Asara to Dizin heights) and northeast of Tehran (Lavasanat). Morphological evaluation was performed according to UPOV instruction (TG 68/3). 32 quantitative and qualitative morphological and horticultural traits were evaluated according to UPOV (TG 3.68). Of which, 11 quantifiable traits including leaf length and width, fruit length and width, pedicel length, Berries per raceme, cluster length, spines length, vitamin C, TA (Titratable Acidity) and TSS (Total Soluble Solid) were analyzed.Results and Discussion The results of analysis of variance showed significant differences between genotypes for all quantative traits evaluated at the level of 1% and for the number of berries per cluster at the level of 5%. The high coefficient of variation indicates high variability for the desired trait, which allows the breeder to have more choices for selecting desired genotypes. The number of fruits per cluster with 45.55, spines length with 28.67 and titratable acid with 26.58 percent malic acid, had the highest range of changes. Qualitative traits included Foliage secondary color, Leaf curvature and Leaf margin, Leaf glossiness, Color of lower side, Fruit tip, Fruit waxiness, Foliage persistence, and Shoot color in spring among the genotypes were uniform and without variance. The lowest coefficient of variation among quantitative traits was related to Brix (7. a16 percent) and fruit length (13.14 percent). The highest number of fruits was belonged to Lavasan genotype (AD8) giving an average of 25 fruits per cluster, which was substantially different from other genotypes. The highest and lowest fruit length and width are related to Taleghan 5 (11.59 mm) and Chalus 7 (3.26 mm) genotypes, respectively. In a study, the average length and width of barberry fruit were 7.69 and 3.32 mm, respectively. Vitamin C is one of the nutritional values of fruits and has direct effects on human health. Wild barberry genotypes possessing the least vitamin C content (4.7 mg/per 100 gram) of fresh fruit (Asara I genotype) and also the fruits containing the highest values (i.e., 10.57 (Taleghan 4), 9.63 (Chalous 8) and 9.4 (Taleghan 8)) yielded more than even temperate fruits such as stone and pome fruits (The mean amount of vitamin C in apples, grapes and black cherries is 4.6, 4, 10 mg/100 g FW, respectively). This value in strawberries as an indicator plant is reported between 10 and 100 and an average of 58.8 and the value for lemons is 53 mg/100 g FW. Khayat and Mahmoud Abadi (2010) reported the amount of vitamin C in seedless barberry treated by fertilizers varied from 4 to 9 mg  per 100 g of fresh fruit.The highest values of total soluble solids or Brix˚ were related to Chalus 8 genotypes with value of 24.83% and Chalus 1 with value of 23.23% and the lowest amount was related to Asara 2 genotype with 18.1%. Khayat and Mahmoud Abadi (2010) reported the total soluble solids ranged between 18.3 to 33.06 percent in seedless barberry, which is much higher than our experiment. The highest titratable acidity were observed in Taleghan 4, Taleghan 8 and Taleghan 2 genotypes, with 2.66, 2.65 and 2.41 mg/ml malic acid respectively, and the lowest titratable acid was observed in Chalus 9 genotype with 1.12 mg/ml malic acid. This value has been reported in domestic barberry is between 1.07 and 2.95. The highest mean leaf lengths were observed in Chalus cultivars 3 and 5. Among the genotypes, Taleghan 7 has the longest Pedicel length.ConclusionThe selected genotypes for breeding programs were the Oshan (AD8) genotypes with an average of 25 fruits per cluster. Regarding vitamin C content the prominent genotypes were the Taleghan 4 (10.57), Chalus 8 (9.63) and Taleghan 8 (9.4) mg/100 g F.W. The highest genotypes for total soluble solids were Chalus 8 genotypes with 24.83 and Chalus 1 with 23.23 percent.