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In-house production of DNA size marker from a vaccinal Bacillus anthracis strain
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نویسنده
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sekhavati m. ,tadayon k. ,ghaderi r. ,banihashemi r. ,jabbari a.r. ,shokri g. ,karimnasab n.
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منبع
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iranian journal of microbiology - 2015 - دوره : 7 - شماره : 1 - صفحه:45 -49
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چکیده
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Background and objectives: dna molecular weight marker is widely used in molecular biology experiments incurring considerable costs on low-budget settings. materials and methods: here a pcr-supported procedure is described that uses 10 primer pairs targeting chromosomal dna from the harmless vaccinal bacillus anthracis sterne 34f2 strain as template. a single pcr protocol is used to reproduce all the 10 fragments of a 100 bp dna size marker. results and conclusion: the unpurified amalgam of 10 pcr products can be directly loaded to agarose gels. this work was intended to develop a reasonably cost-effective dna ladder that is useful for researchers in laboratories with limited funding. © 2015,tehran university of medical science. all rights reserved.
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کلیدواژه
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Bacillus anthracis sterne 34F2; DNA size marker; PCR; Sequencing
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آدرس
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department of veterinary aerobic bacteria,razi institute, ایران, department of veterinary aerobic bacteria,razi institute, ایران, department of veterinary aerobic bacteria,razi institute, ایران, department of veterinary aerobic bacteria,razi institute, ایران, department of veterinary aerobic bacteria,razi institute, ایران, department of veterinary aerobic bacteria,razi institute, ایران, department of veterinary aerobic bacteria,razi institute, ایران
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Authors
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