Isolation,Identification and Antimicrobial Susceptibility of Pantoea (Enterobacter) Agglomerans Isolated From Consumed Powdered Infant Formula Milk (Pif) in Nicu Ward: First Report From Iran

Background and objectives: pantoea agglomerans is a gram-negative rod in the enterobacteriaceae family. it is reported as both commensal and opportunistic pathogen of animals and humans. this organism is potential candidates as powdered infant milk formula-borne opportunistic pathogen. the aim of our study was to perform isolation,identification and antimicrobial susceptibility pattern of pantoea (enterobacter) agglomerans strains isolated from consumed powdered infant formula milk (pif) in nicu ward. materials and methods: a of total 125 powdered infant formula milk (pif) samples were purchased from hospital drug stores between june 2011 to march 2012. p. agglomerans was isolated according to fda method. for final confirmation,biochemical tests embedded in the api-20e system were used. the drug susceptibility test was performed using the disc diffusion method according to clsi guidelines. results: out of the 125 samples investigated,8 (6.4%) samples were positive for p. agglomerans and these were uniformly susceptible to tigecycline,chloramphenicol,cefepime,levofloxacin,minocycline and colistin. fifty percent of isolates were resistant to cefotaxime,moxifloxacin,cotrimoxazole and ticarcillin. conclusion: controlling the primary populations of p. agglomerans during the pif production process and preventing post processing contamination,by using suitable microbiological guidelines,is accessible. sanitary practices for the preparation of infant formula in both the home and hospitals should be carefully controlled.