comparative molecular detection of helicobacter spp. and wolinella in the oral cavity of cats and dogs with periodontal disease and healthy individuals

Background and objectives: helicobacter and wolinella are gram-negative bacteria belonging to the helicobacteraceae family. while helicobacter species are well-known for their role in gastric disorders, emerging evidence suggests their presence in the oral cavity and potential involvement in periodontal diseases.materials and methods: helicobacter and wolinella species were investigated in 122 saliva and periodontal plaque samples from dogs and cats by dna extraction, pcr amplification, and 16s rdna gene identification.results: comparing the periodontitis group and the healthy group, a higher incidence of positive wolinella and helicobacter species was shown in both dog and cat groups. 16s rdna genes of helicobacter were detected in 60% of the cats and 67.7% of the dogs. detection of 16s rdna genes of the wolinella group in felines was 78.3% which was higher than in canines (67.7%). helicobacter felis (35%) was the most common species detected in cats, contrary to dogs, in which helicobacter heilmannii (30%) detection was higher (in both groups). helicobacter pylori was not detected in either group.conclusion: comparing the occurrence of helicobacter and wolinella in the mouths of dogs and cats, their association with periodontal disease, and the possibility of a common source of infections between humans and companion animals is of great importance for the management of oral health in animals and humans.