activity of cefiderocol on extensively drug-resistant pseudomonas aeruginosa from burn wound infections in mansoura, egypt

Background and objectives: increased pseudomonas aeruginosa antibiotic resistance limits treatment options and is associated with a higher level of mortality and mordacity. the purpose of this research was to identify class 1 and 2 integrons, carbapenemase, shv, and tem genes in extensively drug-resistant (xdr) p. aeruginosa isolated from infected burns and evaluate their in vitro cefiderocol activity.materials and methods: by using the disc diffusion method, the antimicrobial susceptibility of 110 p. aeruginosa isolates collected from infected burns were evaluated. xdr p. aeruginosa were screened phenotypically for carbapenemase and extended spectrum β-lactamases (esbls) production. both mic test strip and disc diffusion were employed to test the cefiderocol susceptibility. pcr was used to assess carbapenemase, shv and tem genes and integrons class 1 and 2.results: from the 110 p. aeruginosa, 54 isolates (49%) were xdr. tem gene was detected in 35 isolates. among xdr isolates, carbapenemase genes were detected in 31.5%, with ndm being predominant thirty xdr isolates had class1 integrons. all isolates were sensitive to cefiderocol and its mic50/mic90 was 0.5/1.5mg/l (range 0.064-1.5mg/l).conclusion: nearly half the p. aeruginosa isolates from burn infections were extensively drug-resistant. cefiderocol's in vitro activity demonstrated that it is a promising therapy alternative for treating extensively drug-resistant p. aeruginosa in burn patients.