efficacy of traditional herb aqua extract of teucrium stocksianum and its fractions against hsv-1 virus expression levels of genes (ul46 and us6)

Background and objectives: recently, the anti-herpetic activities of different plant species have been investigated. this study evaluated the effects of teucrium stocksianum aqueous extract on the hsv-1 virus-infected vero cell.materials and methods: the ic50 of the aqueous extract was obtained by the maceration of the plant in boiling water and has been measured with the mtt method, also the q-pcr was used to study viral gene expression reduction.results: results of the mtt test indicated that the highest percentage of metabolic activity was observed in the 75 μg/ml concentration of teucrium stocksianum’s aqueous extracts (ic50=45.5μg/ml). time intervals of 24 and 48 hours after viral infection revealed that the cell viability is reduced by the viral infection time (moi=0.1), log 10-3, p <0.001). furthermore, the plant’s aqueous extract concentration almost avoids cell viability reduction. through q-pcr results; the reduction of viral proliferation revealed that the low expression of genes ul46 and us6 were significant in the presence of different treatments utilized in the experiment.conclusion: t. stocksianum, has an anti-viral property and may be considered as a remedy for anti-hsv-1 agents.