immunogenicity of mannan derived from mycobacterium bovis as a promising adjuvant in vaccine bcg

Background and objectives: lipoarabinomannan is one of the components of the significant structural cell surfaces of mycobacteria and serves as an immunostimulatory factor. tnf-α and il-12 are two examples of the anti-bacterial inflammatory cytokines that are activated and induced during infection.materials and methods: in this study, mannan was extracted and processed, and then bulb/c female mice were used in three groups, one group was given bcg vaccine, the other group was given bcg vaccine with mannan adjuvant, and a non-injected group was used as a control group. inflammatory factors interleukin-12, tnf-α, igg and igm were measured in mouse serum.results: the levels of the inflammatory factors interleukin-12 and tnf-α in the serum isolated from mice receiving the bcg vaccine with mannan adjuvant showed a significant difference compared to the group that received only the bcg vaccine and the control group [il-12] and , with p≤0.05.the examination of the level of igg immune factors in these three groups revealed a significant difference. the group that received the bcg vaccine with mannan adjuvant showed a marked contrast compared to the group that received only the bcg vaccine and the control group, with p≤0.05. the level of igm was higher in the group that received the bcg vaccine alone compared to the adjuvant vaccine group and the control group, with p≤0.05.conclusion: our results indicated that mice receiving the bcg vaccine with mannan adjuvant had significantly higher serum levels of il-12, tnf-α, and igg than the group receiving bcg alone.