secretome of adipose derived-mesenchymal stem cells reduces the vibrio cholerae attachment to caco-2 cells and subsequent inflammatory responses

Background and objectives: mesenchymal stem cells (mscs) can repair gastrointestinal tract damage. the secretome of mscs has a high capacity to inhibit bacterial colonization and the subsequent inflammatory responses of vibrio cholerae.materials and methods: the caco-2 cells were treated with adipose-derived mscs (ad-mscs) secretome and then infected with v. cholerae. subsequently, the bacterial attachment and invasion, cholera toxin gene expression, pge2 and il-6 secretion, tnf-α, il-1β, and il-8 expression, and apoptosis of caco-2 cells were evaluated.results: the secretome of ad-mscs significantly reduced the v. cholerae attachment and internalization on caco-2 epithelial cells (p<0.0001). the cholera toxin (ctx-b) gene expression (fr=4.56 ± 0.66) and pge2 production (p=0.0007) were also significantly reduced. the production of no and tnf-α, il-1β, and il-8 pro-inflammatory cytokines were significantly (p<0.05) reduced in exposure to the secretome of ad-mscs. secretome also improved a significant 81.33% increase in il-6 production (128.1 ± 37.6 pg/ml) and showed a 12.36% significant decrease in epithelial cell apoptosis (p< 0.0001) after exposure to v. cholerae.conclusion: the secretome of ad-mscs can play a critical role in inhibiting bacterial colonization, and subsequent inflammatory responses, and maintaining the integrity of the epithelial barrier. the secretome may be effective in the prevention of hypovolemic shock.