isolation and characterization of a bisphenol a-degrading strain, pseudomonas aeruginosa du2, from soil containing decaying plants

Background and objectives: bisphenol a (bpa) is a toxic compound with broad applications in the plastics industry. bpa has harmful effects on various organisms and its efficient removal is necessary. the microbial degradation of bpa is a safe and economical approach. in this research, soil samples containing decaying plants were screened to isolate a bpa-degrad- able bacterial strain. materials and methods: soil samples were collected from different locations in damghan, semnan province, iran. to enrich bpa-degrading bacteria, the samples were cultured in a stepwise manner in a mineral medium containing increasing bpa concentrations (5 to 40 mg/l). the ability of isolated bacteria in degrading bpa was assayed by folin-ciocalteu and high-performance liquid chromatography methods. the biodegradation efficiency of the most efficient isolate was assayed under distinct conditions and it was identified through the sequencing of the 16s rrna gene. results: among the isolated bacteria, pseudomonas aeruginosa du2 (genbank accession number: op919484) showed the most bpa biodegradation ability. the highest bpa degradation (52.98%) was observed in the mineral medium containing 5 mg/l bpa and the inoculum size of 6 × 10^7 cfu/ml at ph 9 and in the presence of 0.05% (w/v) nacl during 10 days. conclusion: these results offer soil containing decaying plants as a promising source for finding bpa-degrading bacteria. p. aeruginosa du2 has basal bpa removal ability, which could be improved by optimization of medium components and growth conditions.