Type Vi Secretion System (T6ss) in Klebsiella Pneumoniae, Relation To Antibiotic Resistance and Biofilm Formation

Background and objectives: the type vi secretion system (t6ss) was identified as a novel virulence factor in many gram-negative bacteria. this study aimed to investigate the frequency of the t6ss genes in klebsiella pneumoniae-causing different nosocomial infections, and to study the association between t6ss, antibiotic resistance, and biofilm formation in the isolated bacteria.materials and methods: a total of fifty-six non-repetitive k. pneumoniae isolates were collected from different inpatients admitted at sohag university hospital from september 2022 to march 2023. samples were cultured, colonies were identified, and antimicrobial sensitivity was done by vitek® 2 compact. biofilm formation was checked using congo red agar method. t6ss genes, and capsular serotypes were detected by pcr.results: fifty-six k. pneumoniae isolates were obtained in culture. 38 isolates (67.86%) produced biofilm and 44 (78.57%) were positive for t6ss in pcr. there was a significant association between the presence of t6ss and resistance to the following antibiotics: meropenem, ciprofloxacin, and levofloxacin. all biofilm-forming bacteria had t6ss, with significant differences towards t6ss -positive bacteria. there was no significant association between t6ss, and the presence of certain capsular types.conclusion: the t6ss-positive k. pneumoniae has greater antibiotic resistance, and biofilm-forming ability which is considered a potential pathogenicity of this emerging gene cluster.