glucomannan enhanced the macrophage activity in exposure to methicillin-resistant staphylococcus aureus (mrsa): in-vitro study

Background and objectives: the increasing number of methicillin-resistant staphylococcus aureus persuade the need for preventive measures. glucomannan is a polysaccharide choice for developing immunological strategies. this study aimed to investigate changes in gene expression and phagocytic activity of macrophage cells in the presence of glucomannan.materials and methods: the effect of different concentrations of glucomannan (25, 50, and 100 xb5;g/ml) on the phagocytic activity of macrophage cells was measured using the colony count method. the expression of tumor necrosis factor-alpha (tnf-x3b1;) and inducible nitric oxide synthase (inos) genes was evaluated by real-time pcr.results: the concentrations of glucomannan significantly reduced the bacterial colony-forming unit (cfu) and increased the phagocytic activity of macrophage cells. the maximum effect of glucomannan on inos and tnf-x391; genes expression was 100 xb5;g/ml.conclusion: glucomannan should be considered an adjuvant that stimulates the immune system. it may increase the expression of tnf-x3b1; and inos genes and the phagocytic activity of macrophage cells against methicillin-resistant staphylococcus aureus.