prevalence of main quinolones and carbapenems resistance genes in clinical and veterinary escherichia coli strains

Background and objectives: antibiotics-resistant escherichia coli strains are considered one of the most important causes of human and animal infections worldwide. the aim of current study was to detect common resistance (carbapenems and quinolones) genes by pcr. materials and methods: a total of 100 e. coli strains isolated from human urinary tract infection and 20 isolated strains of aborted sheep embryos were collected. pcr was performed using specific primers to detect the resistance genes. results: overall, among the quinolones resistance genes, qnrs resistance gene had the highest frequency (48%) and among carbapenem resistance genes, imp resistance gene had the highest frequency (45%). the frequency of resistance genes, imp (28.45%), kpc (9.5%), vim (9.15%), ndm (7.20%) were observed in clinical and veterinary strains, respectively. accord- ing to the results, 38.6% of e. coli strains had at least one from five genes of resistance to quinolones. the lowest frequency of resistance gene was related to qnra, which was observed in only 29 (24.2%) strains. conclusion: monitoring of carbapenem and quinolone resistance in pathogenic e. coli to humans and animals has an im- portant value in revising treatment guidelines and the national public health, and plays an important role in preventing the spread of resistant strains.