Characterization of the Conserved Regions of E1a Protein From Human Adenovirus For Reinforcement of Cytotoxic T Lymphocytes Responses To the All Genogroups Causes Ocular Manifestation Through An in Silico Approach

Background and objectives: adenovirus species b, c, d, and e are the most common causes of ocular manifestations caused by adenoviruses. fda-approved treatment agents for adenovirus infections are not available. cell-mediated immunity is the major protective mechanism versus human adenoviruses (hadvs) infection and t cells specific for peptide epitopes from nonstructural proteins can prevent adenoviral dissemination. e1a cr2 region of hadvs epitopes predicted for reinforcing cytotoxic t lymphocytes (ctls) in the ekc patients. among human adenoviruses e1 protein, four distinct e1a regions had a significantly higher level of homology than the rest of e1a protein. e1a protein inhibits ifn signal transduction. epitope-based vaccines are designed to have flexible and simple methods to synthesize a vaccine, using an adjuvant to trigger fast immune responses. ctl epitopes were applied to create a multiepitope vaccine. conserve region1 (cr1) and cr3 have less antigenicity compared to cr2. additionally, cr3 in hadv-d8 contains three toxic areas. cr4 similar to the two regions cr1 and cr3 do not show acceptable antigenic properties. materials and methods: bioinformatics’ tools were used to predict, refine and validate the 3d structure of the construct. effective binding was predicted by protein-protein docking of the epitope vaccine with mhc-i molecules and revealed the safety and efficacy of the predicted vaccine construct. results: in silico analysis show that rising levels of cytotoxic cd8 + t cells, th1 cells, macrophages, and neutrophils are linked to ifn-dominant th1-type responses, which are detected in putative immune individuals. conclusion: combined with 3d protein modeling, this study predicted the epitopes of e1a cr2 protein in hadvs.