Lytic Potential of A Filamentous Bacteriophage Isolated From Sewage Water in Tehran on Clinical Carbapenem-Resistant Strains

Background and objectives: acinetobacter baumannii (a. baumannii), an opportunistic pathogen, has been isolated from sewage, soil, and hospital wards. the prevalence of multidrug-resistance a. baumannii has seriously caused a health crisis in hospital settings. bacteriophages have been used as an alternative therapy for control carbapenem-resistant bacteria-caused infections. we aimed to assay lytic effect of a filamentous phage on clinical bacterial strains. materials and methods: 500-ml water samples was collected from sewage in tehran. sewage samples were precipitated at 6000 rpm for 10 minutes and filtered using 0.45-μm syringe filters. bacteriophage was isolated using double-layer agar assay and evaluated its stability at various ph and temperature ranges. in addition, the stability of the phage was assayed at chloroform 0.1%. results: transmission electron microscopy imagine showed phage is filamentous called vb-abai-tmu2. the phage affected on its own host so that could not effect on any pseudomonas aeruginosa and klebsiella pneumoniae strains. vb-abai- tmu2 phage was stable at ph 5 and 7 and also temperatures 25 and 37°c. vb-abai-tmu2 was stable at chloroform 0.1%. vb-abai-tmu2 phage infected carbapenem-resistant a. baumannii strains while other bacterial strains were resistant to. conclusion: the present study indicated the isolated phage had a narrow host range and is susceptible to various ph values and temperatures.