Efficacious Antibacterial Potency of Novel Bacteriophages Against Esbl-Producing Klebsiella Pneumoniae Isolated From Burn Wound Infections

Background and objectives: prevalence of extended spectrum β-lactamase (esbl) leads to the development of antibiotic resistance and mortality in burn patients. one of the alternative strategies for controlling esbl bacterial infections is clinical trials of bacteriophage therapy. the aim of this study was to isolate and characterize specific bacteriophages against esbl-producing klebsiella pneumoniae in patients with burn ulcers. materials and methods: clinical samples were isolated from the hospitalized patient in burn medical centers, iran. biochemical screenings and 16s rrna gene sequencing were determined. the phages were isolated from municipal sewerage treatment plants, isfahan, iran. tem and fesem, adsorption velocity, growth curve, host range, and the viability of the phage particles as well as proteomics and enzyme digestion patterns were examined. results: the results showed that klebsiella pneumoniae iaufa_lad2 (genbank accession number: mw836954) was confirmed as an esbl-producing strain using combined disk method. this bacterium showed significant sensitivity to three phages including pɸbw-kp1, pɸbw-kp2, and pɸbw-kp3. morphological characterization demonstrated that the phage pɸbw-kp3 to the siphoviridae family (lambda-like phages) and both phages pɸbw-kp1 and ɸbw-kp2 to the podoviridae family (t1-like phages). the isolated bacteriophages had a large burst size, thermal and ph viability and efficient adsorption rate to the host cells. conclusion: in present study, the efficacy of bacteriophages against esbl pathogenic bacterium promises a remarkable achievement for phage therapy. it seems that, these isolated bacteriophages, in the form of phage cocktails, had a strong antibacterial impacts and a broad-spectrum strategy against esbl-producing klebsiella pneumoniae isolated from burn ulcers.