A Novel Method For the Extraction of Outer Membrane Vesicles (Omvs) From Bordetella Pertussis Tohama Strain

Background and objectives: there are many pertussis outbreaks which is mainly due to the reduction in the immunity of acellular pertussis (ap) vaccines. therefore, there is a crucial necessity to develop a new generation of pertussis vaccine. preceding researches have shown that bordetella pertussis outer membrane vesicles (omvs) have appropriate specifications, making them a suitable vaccine candidate against pertussis. materials and methods: the omvs were separated by a new serial ultra centrifugation technique. transmission electron microscopy (tem) examination, sdspage, western blotting and elisa assay were used to characterize the omvs. results: tem studies showed the size of the extracted omvs at 40200 nm. the presence of pertussis toxin, filamentous hemagglutinin, and pertactin was verified using western blot and elisa assay. conclusion: the presented technique is a simple and effective way to obtain omvs from bordetella pertussis. so it can be utilized as an appropriate procedure in the development of an omvbased vaccine against pertussis.