immune response in cutaneous leishmaniasis patients with healing vs. non-healing lesions

Background and objectives: the outcome of leishmania infection mainly depends upon the leishmania species whichcauses the disease and the generation of the type of host immune response, the healing process and protection in leishmaniasisdepends upon induction of th1 response. in this study, the th1/th2 cytokine profile in cutaneous leishmaniasis (cl)is evaluated.materials and methods: this study was carried out in leishmaniasis clinic of crtsdl, tums, during march 2018 tomarch 2019. peripheral blood mononuclear cells (pbmc) of volunteers with active healing and non-healing lesion (s) ofcutaneous leishmaniasis (cl), volunteers with and without history of cl were cultured and stimulated with soluble leishmaniaantigen (sla). the supernatants were collected and the levels of ifn-γ, il-5 and il-10 were titrated using elisamethod.results: the results showed a significantly higher levels of ifn-γ in volunteers with active cl healing form (p<0.005),history of cl (p<0.005) than healthy volunteers. a significantly (p<0.005) higher level of ifn-γ was seen in volunteers withactive healing form of lesion than non-healing form. there was a significantly (p<0.005) higher level of il-10 in volunteerswith a history of non-healing form and active non-healing form of cl. there was no significant difference in il-5 productionin pbmc of different groups.conclusion: ifn-γ production starts at early stage of cutaneous leishmaniasis and enhance during course of lesion healing,ifn-γ level is significantly higher in all patients compared to healthy volunteers, ifn-γ is significantly higher in patients withhealing form than non-healing form of lesion.