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Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex
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نویسنده
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Ghodousi A ,Vatani S ,Darban-Sarokhalil D ,Omrani M ,Fooladi AA ,Khosaravi AD ,Feizabadi MM
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منبع
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iranian journal of microbiology - 2012 - دوره : 4 - شماره : 1 - صفحه:43 -45
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چکیده
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A modified pulsed-field gel electrophoresis (pfge) protocol was developed and applied to clinical isolates of mycobacterium tuberculosis complex to reduce the cost of using lyticase. this protocol reduces the expense of pfge typing of mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria
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کلیدواژه
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Mycobacterium tuberculosis ,TB-complex ,Pulsed-filed gel electrophoresis
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آدرس
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tehran university of medical sciences tums, School of Medicine, Department of Microbiology, ایران, ahvaz jundishapur university of medical sciences, School of Medicine & Infectious and Tropical Diseases Research Centre, Department of Microbiology, ایران, tehran university of medical sciences tums, School of Medicine, Department of Microbiology, ایران, tehran university of medical sciences tums, School of Medicine, Department of Microbiology, ایران, baqiyatallah university of medical sciences, School of Medical Sciences, Research Center of Molecular Biology, Department of Pathology, ایران, ahvaz jundishapur university of medical sciences, School of Medicine & Infectious and Tropical Diseases Research Centre, Department of Microbiology, ایران, tehran university of medical sciences tums, School of Medicine, Department of Microbiology, ایران
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پست الکترونیکی
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mfeizabadi@tums.ac.ir
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Authors
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