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Dye Labelled Monoclonal Antibody Assay For Detection of Toxic Shock Syndrome Toxin -1 From Staphylococcus Aureus
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نویسنده
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Javid Khojasteh V. ,Foster Ha
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منبع
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Iranian Journal Of Microbiology - 2011 - دوره : 3 - شماره : 4 - صفحه:170 -176
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چکیده
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Objective: the aim of study was to develop a rapid assay, dye labelled monoclonal antibody assay (dlmaa), using non-radioactive organic synthetic dyes for identification of toxic shock syndrome toxin-1 (tsst-1) producing strains of staphylococcus aureus.materials and methods: the assay protocol required only two simple steps; addition of tsst-1 antigen to a nitrocellulose membrane and then adding a colloidal dye labelled antibody (d/a) suspension detection reagent.results: the sensitivity and specificity of the assay was determined relative to positive and negative strains compared to an elisa assay. overall 100% agreement was found between both assays. the sensitivity for detection of tsst-1 was 30 ng.conclusion: the dlmaa did not require handling and disposal of radioactive materials. it is a rapid qualitative technique for detection of tsst-1 toxin at room temperature within a short time.
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کلیدواژه
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Monoclonal Antibody ,Staphylococcus Aureus ,Tsst-1 ,Colloidal Dye ,Elisa
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آدرس
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Guilan University Of Medical Sciences, Faculty Of Shahid Beheshty, Department Of Bacteriology, ایران, University Of Salford, School Of Environment And Life Sciences, Center Of Parasitology And Disease Reserch, Uk
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پست الکترونیکی
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tosan_123@yahoo.co.uk
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Authors
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