the differences in systemic humoral immune response among balb/c mice administered with probiotic, lps escherichia coli, and probiotic-lps e. coli

Background and objectives: the aim of this study was to compare the systemic humoral immune responses, including ige, iga, igg and igm levels in balb/c mice administered a probiotic, lps derived from escherichia coli (e. coli), and probiotic-lps derived from e. coli. materials and methods: thirty-two male balb/c mice, 10-12 weeks of age with body weight ranging from 30-40 g were randomly divided into four experimental groups (n=8). the treatment regimens were as follows: group 1, mice did not receive lps or probiotic (control group); group 2, mice received only lps on the first day; group 3, mice received probiotic for 7 days; group 4, mice received lps on the first day, and then continued, with probiotic for 7 days. the mice were observed for 8 days, and then, euthanized the next day (day 9). the serum was collected, and the levels of ige, iga, igg and igm were measured using elisa. results: the humoral immune response was higher in the presence of a probiotic compared to that in the control; ige (9.02 ± 0.58 units/ml, p=0.000), iga (3.26 ± 0.99 units/ml, p=0.316), igg (7.29 ± 0.24 units/ml, p=0.000), and igm (4.01 ± 2.98 units/ml, p=0.505). when administered with lps e. coli along with probiotic, the humoral immune response was the highest; ige (10.68 ± 1.63 units/ml, p=0.000), iga (8.34 ± 1.47 units/ml, p=0.000), igg (9.96 ± 0.98 units/ml, p=0.000), and igm (4.31 ± 1.05 units/ml, p=0.319) compared to the control group. conclusion: probiotic-lps derived from e. coli treatment induced a higher humoral immune response (highest ige, iga, igg and igm levels) compared to treatment with probiotic only