comparative effects of granulocyte-colony stimulating factor and colistin-alone or in combination on burn wound healing in acinetobacter baumannii infected mice

Background and objectives: burn wounds are one of the most important health problems all over the world because infection after burn can delay wound healing. treating burn wounds with granulocyte-colony stimulating factor (g-csf) is known to improve healing of injured tissue. in addition, colistin is prescribed as an effective treatment. the aim of this study was to evaluate the effect of g-csf and colistin alone or in combination with g-csf on wound healing of acinetobacter baumannii (a. baumannii) infected burns. materials and methods: this study was performed between january 2016 and april 2018. burn wounds were experimentally induced in 36 mice. the wounds were inoculated with a. baumannii. in a 7-day period, burn wounds in each group were daily treated with subcutaneous injections (0.1 ml) of saline, g-csf, colistin, and g-csf plus colistin. after killing the animals, the size of the wound, number of leukocytes in the skin and microbial growth were evaluated. a value of p ≤ 0.05 was considered statistically significant. results: wound healing in the g-csf plus colistin group was significantly higher than the control group and the g-csf group (p = 0.023 and p = 0.033, respectively). in g-csf+colistin group, the number of leukocytes was higher than the control group considerably (p = 0.007). on the 7th day of treatment, number of positive bacterial cultures in the colistin and the g-csf plus colistin groups was lower than other groups with a significant difference. conclusion: concurrent consumption of g-csf and antibiotics can control burn infection and enhance the immune system towards wound healing.