Influence of Zno Nanoparticles on Candida Albicans Isolates Biofilm Formed on the Urinary Catheter

Background and objectives: the aim of this study was to determine the effect of zinc oxide nanoparticle (zno-np) solution in the surface catheter on c. albicans adhesion and biofilm formation. materials and methods: out of 260 isolates from urinary catheter, 133 were determined as c. albicans by common phenotypic and genotyping methods. zno nanoparticles with 30 nm were made by the sol-gel method, which was confirmed by xrd (x-ray diffraction) and scanning electron microscope (sem) methods. candidal adhesion and biofilm assays were performed on catheter surfaces for 2 and 48 hours, respectively. the effect of sub-mic (minimum inhibitory concentrations) and mic concentrations of zno-np on biofilm formation was evaluated after 24 hours using crystal violet (cv), colony-forming unit (cfu), and sem. results: out of 133 c. albicans isolates, 20 (15%) fluconazole-resistant and 113 (85%) susceptible isolates were determined by the disk diffusion method. results showed that both isolates adhered to biofilm formation on the catheter surfaces. a significantly (p< 0.05) higher number of cfus was evident in fluconazole-resistant biofilms compared to those formed by susceptible isolates. zno-np reduced biofilm biomass and cfus of dual isolate biofilms (p< 0.05). zno nanoparticles had a significantly (p< 0.05) greater effect on reducing fluconazole-resistant c. albicans biofilm biomass compared to susceptible isolates. conclusion: zno-np exhibits inhibitory effects on biofilms of both isolates. these findings provide an important advantage of zno that may be useful in the treatment of catheter-related urinary tract infection.