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On-Column Approach in the HPLC-UV Analysis of Non-chromophoric Compounds Using Azelaic Acid as a Model
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نویسنده
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Alzweiri Muhammed ,Al-Hiari Yusuf M. ,Aburjai Talal ,Abdel-Aldaem Osama
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منبع
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jordan journal of pharmaceutical sciences - 2012 - دوره : 5 - شماره : 3 - صفحه:243 -251
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چکیده
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The manuscript introduces a detection solution of compounds lacking chromophoric properties (e.g. azelaic acid)by implementing a hplc-uv analysis with on-column derivatisation of the analyte. azelaic acid was used totest the feasibility of the method at λmax = 265 nm. its chromatographic analysis shows linear (r = 0.999),precise (rsd < 2.0%) and accurate (97.0 - 103.5 %) behavior. furthermore, the method was found selective forazelaic acid in a prepared cream which contains other ingredients such as triethanolamine, vaseline and stearicacid. the limit of detection (lod) and limit of quantification (loq) of azelaic acid were 9 and 30 μg/ml,respectively
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کلیدواژه
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HPLC-UV ,Azelaic acid ,On-column derivatisation
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آدرس
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University of Jordan, Faculty of Pharmacy, Department of Pharmaceutical Sciences, Jordan, University of Jordan, Faculty of Pharmacy, Department of Pharmaceutical Sciences, Jordan, University of Jordan, Faculty of Pharmacy, Department of Pharmaceutical Sciences, Jordan, University of Jordan, Faculty of Pharmacy, Department of Pharmaceutical Sciences, Jordan
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Authors
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