The Stimulatory Effect of MIP-1alphathe Gene Transduction Efficacy of Cord Blood CD34+ Cells by a Pseudotype Retroviral Vector

Introduction: hematopoietic stem cells are always in a quiescence state. since they need retroviral transduction to infect dividing cells, they are resistant to retrovirus transduction. they need to be pre-stimulated by acytokine cocktail. for proliferation without maturation, we suggest mip-1ل as a novel factor. material and methods: retroviral vector produced by pg13/ln c8 cells titter on hela cells. then, the cd34+ cells of cord blood can be pre-stimulated in a serum- free media supplemented with scf, flt3,tpo,il6 in the presence and absence of 50 ng/ml mip-1ل. transduction efficiency was assessed by a semi-quantitative pcrfor the neomycin gene. results: a pcr analysis of the neomycin gene in cd34+ cells revealed an improved transduction of cord bloodcells in the presence of mip-1ل 65%, in comparison to its absence: 40.7%. conclusion: the addition of mip-1ل to the cytokine cocktail improves the transduction efficiency of cord blood hematopoietic progenitor cells. further studies are required to clarify its effect on the functional properties of cd34+ cells.