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thalidomide is more efficient than sodium butyrate in enhancing gata-1 and eklf gene expression in erythroid progenitors derived from hscs with β-globin gene mutation
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نویسنده
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jalali far mohammad ali ,dehghani fard ali ,hajizamani saiedeh ,mossahebi-mohammadi majid ,yaghooti hamid ,saki najmaldin
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منبع
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international journal of hematology-oncology and stem cell research - 2016 - دوره : 10 - شماره : 1 - صفحه:37 -41
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چکیده
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background: efficient induction of fetal hemoglobin (hbf) is considered as an effective therapeutic approach in beta thalassemia. hbf inducer agents can induce the expression of γ-globin gene and produce high levels of hbf via different epigenetic and molecular mechanisms. thalidomide and sodium butyrate are known as hbf inducer drugs.
material and methods: cd133+ stem cells were isolated from umbilical cord blood of a newborn with minor β-thalassemia in order to evaluate the effects of these two drugs on the in vitro expression of gata-1 and eklf genes as erythroid transcription factors. cd133+ stem cells were expanded and differentiated into erythroid lineage, and then treated with thalidomide and sodium butyrate and finally analyzed by quantitative real-time pcr. statistical analysis was performed using student’s t-test by spss software.
results: thalidomide and sodium butyrate increased gata-1 and eklf gene expression, compared to the non-treated control (p<0.05).
conclusion: thalidomide was more efficient than sodium butyrate in augmenting expression of gata-1 and eklf genes. it seems that gata-1 and eklf have crucial roles in the efficient induction of hbf by thalidomide. |
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کلیدواژه
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fetal hemoglobin ,thalidomide ,sodium butyrate ,β-thalassemia
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آدرس
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ahvaz jundishapur university of medical sciences, ایران, sarem cell research center-scrc, sarem women’s hospital, tehran, iran, Iran, Islamic Republic of, shiraz university of medical sciences, ایران, tarbiat modares university, ایران, ahvaz jundishapur university of medical sciences, ایران, ahvaz jundishapur university of medical sciences, ایران
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پست الکترونیکی
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najmaldinsaki@gmail.com
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Authors
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