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in vitro effects of propranolol on t helper type 1 cytokine profile in human
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نویسنده
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hajighasemi fatemeh ,mirshafiey abbas
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منبع
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international journal of hematology-oncology and stem cell research - 2016 - دوره : 10 - شماره : 2 - صفحه:99 -105
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چکیده
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introduction: cytokines are a large group of proteins play a key role in inflammation. down-regulation of pro-inflammatory cytokines has beneficial effect on heart function. propranolol, as a non selective beta-adrenergic blocker, has been extensively used for treatment of many cardiovascular problems such as arrhythmias and heart malfunction. in addition anti-inflammatory effects of propranolol have been revealed. in this study the propranolol effect on t helper type 1 cytokine profile in human leukemic t cells has been assessed in vitro.
materials and methods: human leukemic t cells (molt-4 and jurkat) were cultured in complete rpmi medium. the cells were then incubated with different concentrations of propranolol (0.03- 30 µm) in the presence or absence of pha (10 µg/ml) for 48 hours. the supernatants of cell culture media were collected and used for cytokines assay.
results: propranolol significantly decreased the t helper type 1 cytokine profile [interleukin-2 (il-2) and interferon- γ (ifn-γ)] production in pha stimulated molt-4 and jurkat cells, after 48 hour of incubation time, dose-dependently compared to untreated control cells.
conclusion: our data showed a dose dependent inhibitory effect of propranolol on the il-2 and ifn-γ production in human leukemic molt-4 and jurkat cells. the anti- inflammatory effect of propranolol reported by other investigators may be in part due to its suppressive effect on production of inflammatory cytokines such as il-2 and ifn-γ. so, propranolol along with its chronic long-term usage in cardiovascular problems may have potential implication in treatment of inflammatory-based disorders.
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کلیدواژه
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propranolol ,cytokines ,leukemic ,t-cells
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آدرس
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shahed university, ایران, department of pathobiology, school of public health, tehran university of medical sciences, tehran, iran., Iran, Islamic Republic of
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پست الکترونیکی
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mirshafiey@tums.ac.ir
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Authors
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