|
|
innovating glioma therapy using secretions from umbilical cord mesenchymal stem cells to target homeobox and growth factor genes
|
|
|
|
|
نویسنده
|
faried ahmad ,adam achmad ,widowati wahyu ,sutendi annisa firdaus ,nindya faradhina salfa ,saputro william junino ,hadiprasetyo dhanar septyawan
|
منبع
|
international journal of hematology-oncology and stem cell research - 2025 - دوره : 19 - شماره : 1 - صفحه:17 -28
|
چکیده
|
Background: glioblastoma is a prevalent and challenging malignant brain tumor. secretome therapy using human umbilical cord mesenchymal stem cells (hucmscs) appears to be a promising treatment for glioblastoma. this study analyzed the potential of the hucmsc secretomes (hucmscs-sec) for glioma therapy.materials and methods: characterization of hucmscs was performed by examining certain markers, including cd44, cd90, cd105, cd73, cd13, cd19, cd14, cd45, cd34, and hla-d. the cells' ability to differentiate into adipocytes, chondrocytes, and osteocytes was evaluated. cytotoxic effect on glioblastoma (gbm) cells was analyzed using 2-[2-methoxy-4-nitrophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2h-tetrazolium (wst-8). mrna relative expression, including homeobox (hoxa5, hoxb1, hoxc9 and hoxc10), insulin-like growth factor binding protein 2 (igfbp2), extracellular signal-regulated kinases (erk), epidermal growth factor receptor (egfr), and caspase 3 (casp3), were quantified by quantitative reverse transcription polymerase chain reaction (qrt-pcr).results: the hucmscs-sec was successfully isolated and identified, showing positive markers and its capacity to differentiate into chondrocytes, adipocytes, and osteocytes. hucmscs-sec exerted a cytotoxic effect on gbm cells and upregulated the expression of casp3, whereas it decreased the expression of hox, igfbp2, egfr, and erk in gbm cells.conclusion: the secretomes from hucmscs show potential for gbm cell therapy by improving the deregulation of hox, inducing apoptosis, and inhibiting cell proliferation genes.
|
کلیدواژه
|
cytotoxic; mrna expression; glioblastoma therapy; human umbilical cord mesenchymal stem cells (hucmscs); secretome
|
آدرس
|
padjadjaran university, faculty of medicine, department of neurosurgery, indonesia, dr. hasan sadikin hospital, oncology and stem cell working group, indonesia. padjadjaran university, faculty of medicine, department of neurosurgery, indonesia, maranatha christian university, faculty of medicine, indonesia, biomolecular and biomedicine research center, aretha medika utama, indonesia, biomolecular and biomedicine research center, aretha medika utama, indonesia, universitas pendidikan indonesia, faculty of mathematics and natural sciences education, biology study program, indonesia, universitas jenderal achmad yani, faculty of pharmacy, indonesia
|
پست الکترونیکی
|
dhanar3095@gmail.com
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|