involvement value of flt-3, c-myc, stat3, p27, and hotair gene expression in acute myeloid leukemia patients: a molecular perspective to a novel leukemogenesis mechanism

The identification of long non-coding rnas (lncrnas) in the pathogenesis of acute myeloid leukemia (aml) has marked a new era in the molecular understating of the disease. this study investigated the correlation between the changes in the expression of lncrnas, including hotair, pvt-1, and crnde, and the alteration in the expression profile of flt-3, c-myc, stat3, stat5, and p27 in aml patients. materials and methods: blood samples were collected from forty-one newly diagnosed aml patients and ten healthy individuals to evaluate the expression levels of the study genes using qrt-pcr analysis. the probable correlation between the gene expressions was determined using pearson’s correlation test. results: the results showed that while there was a significant elevation in the expression of flt3, c-myc, stat3, and hotair, p27 expression remarkably diminished in aml patients compared to the control group. also, a correlation was found between the expression of flt-3 and p27 and the expression of hotair and stat3. it was assumed that flt-3 had a role in increasing the proliferative and survival capacity of aml cells, at least partly, through c-myc-mediated suppression of p27. moreover, lncrna hotair showed to be involved in leukemia proliferation assumably by enhancing the expression of stat3. conclusion: overall, the results of gene profile analysis suggested that studying the expression of hotair, flt-3, c-myc, stat3, and p27 could be helpful to aml patients, and each of these genes could be a valuable target for pharmaceutic intervention.