PCR-RFLP Is a Useful Tool to Distinguish between C. Dubliniensis and C. Albicans in Cancer Patients in Iran

Introduction: candida dubliniensis and c. albicans are very similar in morphology and phenotypic characteristics. approximation of this yeast species has caused major problems in identifying these two correctly.materials and methods: to distinguish among sixty yeast clinical isolates from patients with cancer, polymerase chain reaction - restriction fragment length polymorphism (pcr-rflp) was done.results: pcr-rflp of the its region showed different patterns between candida dubliniensis and c. albicans after digestion with enzymes blni. all of the clinical isolates were diagnosed as c .albicans. the results were confirmed by sequencing and rapd-pcr.conclusion: pcr-rflp would be a useful and applicable technique in clinical laboratories for discrimination of c. albicans and c. dubliniensis.