colloidal gold assay, a new screening test for detection of rsv infection: a controlled trial

Background: respiratory syncytial virus (rvs) is the main cause of morbidity and mortality in the pediatric population. objectives: the objective of the study was to compare the diagnostic accuracies of the colloidal-gold-genesis and newly developed real-time rt-pcr-ingenigen with an admitted real-time rt-pcr-huaruian as the gold standard for respiratory syncytial virus detection. methods: these three assays were performed on collected specimens. results: altogether 258 specimens were studied. among them, real-time rt-pcr-huaruian detected the respiratory syncytial virus in 166 samples, while colloidal-gold-genesis detected 171 samples and real-time rt-pcr-ingenigen detected 168 samples as positive. the overall sensitivity for the detection of the respiratory syncytial virus in comparison to real-time rt-pcr-huaruian was 95.2% for colloidal-gold-genesis and 100% for real-time rt-pcr-ingenigen. the specificity was 85.9% for colloidal-gold-genesis and 97.8% for real-time rt-pcr-ingenigen. the positive likelihood ratio was 6.75 for colloidal-gold-genesis and 45.45 for real-time rt-pcringenigen. the negative likelihood ratio was 0.06 for colloidal-gold-genesis and 0.00 for real-time rt-pcr-ingenigen. the cycle threshold value of real-time rt-pcr-ingenigen was higher than that of real-time rt-pcr-huaruian. conclusions: colloidal-gold-genesis is a good alternative for fast screening of respiratory syncytial virus detection.