اثر دوز های مختلف نیکوتین بر شاخص میتوزی اپی تلیوم مری

Introduction & objective: nicotine could directly act as a cancer promoter. the purpose of this study was to evaluate effects of nicotine on mitotic index in esophagus epithelium.materials & methods: in the present study 30 adult male mice were used. animals were ran-domly divided into three groups. group a or the control group received vehicle, groups b and c received nicotine intraperitoneally at doses of 0.2 and 0.4 mg/kg once daily for 14 days, re-spectively. evaluations were made using ki-67 immunohistochemistry and hematoxilin& eo-sin for proliferative activity and morphometric study on esophagus mucosa, respectively.results: administration of nicotine in group c, showed a significant increase (p < 0.05) in ki-67 index 34.15±2.50vs. 10.41±1.4 compared with the control subjects. the other parameters such as epithelial height, lamina propria, muscular mucosa and mucosa height in nicotine- treated groups were not affected. nicotine at dose of 0.2 mg/kg did not change the mitotic index in epithelium when compared with the control group.conclusion: this study indicates nicotine at dose of 0.4 mg/kg increases mitotic activity in basal cells in esophagus epithelium. (sci j hamadan univ med sci 2016; 23 (2):126-133)