Cellulase Production by Trichoderma reesei using Sugar Beet Pulp

Cellulase production by the fungus trichoderma reesei was studied usingsugar beet pulp (sbp) as a substrate. the subculture medium was a salt solution consisting ofkh2po4, cacl2, etc. fungal cells were sub-cultured in an orbital shaker (180 rpm) at 30°cfor 1-2 generations (two days for each generation) and were then used as an inoculum.exponential cells were inoculated into a medium containing sbp. data showed that cellulosein sbp has a potential to be used as a substrate to produce cellulase. the effect of celluloseconcentration on cellulase production was investigated. using the medium composition (themixture of chemical salts and sbp substrate) and a fermentation time of 4-6 days, amaximum cellulase activity of 0.46 iu/ml of filter paper activity was obtained.