بررسی ارتباط چندشکلی foki ژن xrcc3 با خطر ابتلا به سرطان پستان در زنان: گزارش کوتاه

زمینه و هدف: سرطان پستان یکی از شایع ترین سرطان ها در زنان سرتاسر دنیا است. میزان شیوع سرطان پستان در زنان ایرانی در حال افزایش است. عوامل خارجی و داخلی مختلفی بر روی ثبات ژنوم و خطر ابتلا به سرطان پستان تاثیر دارند. چندشکلی های تک نوکلئوتیدی موجود در ژن های تعمیری dna با افزایش خطر ابتلا به سرطان پستان در ارتباط هستند. هدف از مطالعه حاضر بررسی ارتباط بین خطر سرطان پستان و پلی مورفیسم foki در ژن xrcc3 بود.روش بررسی: این مطالعه موردی شاهدی در بازه زمانی مهر 1395 تا اسفند 1396 در زنان مبتلا به سرطان پستان و زنان سالم واقع در استان مرکزی در آزمایشگاه تحقیقاتی دانشگاه اراک، شهر اراک انجام شد. در این مطالعه ارتباط پلی مورفیسم foki ژن xrcc3 با خطر ابتلا به سرطان پستان در زنان با استفاده از روش هضم آنزیمی pcr-rflp مورد بررسی قرار گرفت.یافته ها: اختلاف معناداری بین دو گروه بیمار و کنترل برای سه ژنوتیپ جایگاه rs1799794 مشاهده نشد (0/435=p).نتیجه گیری: ارتباط معناداری میان چندشکلی foki در ژن xrcc3 و خطر ابتلا به سرطان پستان وجود نداشت.

Study of the association FokI polymorphisms of the XRCC3 gene with the risk of breast cancer in women: brief report

Background: Breast cancer is one of the most common worldwide malignancies among women. Biological data suggest that damage induced by endogenous and exogenous factors affects the integrity of DNA and associated with susceptibility to breast cancer. Single nucleotide polymorphisms (SNPs) in DNA repair genes can associated with differences in the repair efficiency of DNA damage and may affect breast cancer. The XRCC3 protein participates in DNA doublestrand breaks and recombinational repair, in other words the product of XRCC3 gene, plays a key role in homologous recombination repair of DNA doublestrand breaks. The polymorphism of FokI plays critical roles in breast cancer development. The aim of the present study was to evaluate associations between the risk of breast cancer and FokI polymorphism in the XRCC3 gene.Methods: This casecontrol study was carried out on the women with breast cancer and healthy women located in Markazi province at Arak University Research, Iran, from October 2016 to March 2017. In the present study, the association of FokI polymorphisms and the risk of breast cancer was assessed by Polymerase chain reactionrestriction fragment length polymorphism (PCRRFLP) techniques. In this method, genomic DNA was extracted from blood samples using the kit procedure. Then, PCR was performed and the SNPcontaining DNA amplicons were subjected to digestion of enzymes. Following digestion, each sample was immediately analyzed by 3% agarose gel electrophoresis. Statistical analysis was done using SPSS and SNP Analyzer softwares and the final results were determined.Results: No statistically significant difference was observed between the two groups of patients and controls for three genotypes the site rs1799794 (P=0.435). Genotype AG (P=0.384, OR=0.614, CI=95%, 0.2051.840) and GG (P=0.867, OR=0.911, CI=95%; 0.3082.699) had no significant associations with risk of breast cancer.Conclusion: There was no significant association between FokI polymorphisms of the XRCC3 and risk of susceptibility to breast cancer, which was in accordance to some researchers. FokI polymorphisms of XRCC3 gene cannot be used as a biomarker in clinical predictive studies in relation to risk of breast cancer.