molecular analysis of rs2070744 and rs1799983 polymorphisms of nos3 gene in iranian patients with multiple sclerosis

Introduction: multiple sclerosis (ms) is a disease of central nervous system that mainly causes lesions or plaques in the spinal cord and brain. the purpose of this study was to analyze the relation between c.-813c>t (rs2070744) and c.894g>t (rs1799983) polymorphisms of nos3 gene and ms in iranian patients. methods: a total of 78 patients with ms and 80 healthy controls were screened for nos3 (rs2070744 and rs1799983) single nucleotide polymorphisms (snps) by tetra-primer multiplex arms-pcr and pcr-rflp. results: genotype frequencies of the c.-813c>t polymorphism in patients compared to controls were as follows: 53.8% to 80.0% for tt genotype, 41.0% to 18.8% for tc genotype, and 5.1% versus 1.2% for cc genotype (p=0.001). the frequencies of gg genotype was 57.7% and 78.8% and for gt genotype of c.894g>t polymorphism in patients compared to control subjects was 42.3% and 21.2%, respectively (p=0.004). conclusion: our results indicate that the studied nos3 polymorphisms may be associated with ms in iranian patients.