intranasal autologous conditioned serum attenuates memory impairment after mpfc ischemia in mice

Introduction: globally, stroke ranks as the second most prevalent cause of death, contributing significantly to worldwide mortality burdens, imposing a significant economic and emotional challenge on societies. this study was designed to investigate the effect of autologous conditioned serum (acs) on memory and associated molecular factors in a mouse model of photothrombotic ischemic stroke.methods: the photothrombotic model was used to induce medial prefrontal cortex (mpfc) ischemia. acs were prepared by intracardiac puncture of c57bl/6 mice using special acs syringes. after blood incubation, the sample was centrifuged, and the serum was analyzed with elisa kits to quantify the levels of interleukin-1 receptor antagonist (il-1ra) and insulin-like growth factor (igf-i). the ischemic animals received 48 µl intranasal acs two times a day, once a day, or once every other day for one week. behavioral tests, including the lashley-iii maze and social interaction test, were conducted following treatment administration. additionally, igf-1, il-1β, il-1ra levels, and phospho-tau/total-tau ratio were measured in the mpfc area by western blot. histological analysis was performed to assess ischemic volume.results: the results indicated that once-daily administration of acs significantly improved spatial memory in the lashley-iii maze and showed a notable enhancement in social memory as measured by the social interaction test. in terms of molecular analysis, acs increased the levels of igf-1 and il-1ra, whilst decreasing the levels of il-1β and p-tau/total-tau ratio.conclusion: in conclusion, post-stroke intranasal acs administration enhances memory, possibly by increasing the level of igf-1 and attenuating inflammation through the inhibition of il-1β signal by il-1ra, and regulation of tau levels.