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Transdifferentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Dopaminergic Neurons in a Three-Dimensional Culture
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نویسنده
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moayeri ardeshir ,alizadeh rafieh ,ghasemi hamidabadi hatef ,nazm bojnordi maryam ,niapour ali ,hedayatpour azim ,darvishi marzieh ,heidari fatemeh ,soleimani maryam ,elyasi leila
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منبع
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basic and clinical neuroscience - 2022 - دوره : 13 - شماره : 5 - صفحه:625 -636
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چکیده
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Introduction: the induction of human umbilical cord-derived mesenchymal stem cells (huc-mscs) toward dopaminergic neurons is a major challenge in tissue engineering and experimental and clinical treatments of various neurodegenerative diseases, including parkinson disease. this study aims to differentiate huc-mscs into dopaminergic neuron-like cells. methods: following the isolation and characterization of huc-mscs, they were transferred to matrigel-coated plates and incubated with a cocktail of dopaminergic neuronal differentiation factors. the capacity of differentiation into dopaminergic neuronlike cells in 2-dimensional culture and on matrigel was assessed by real-time polymerase chain reaction, immunocytochemistry, and high-performance liquid chromatography. results: our results showed that dopaminergic neuronal markers’ transcript and protein levels were significantly increased on the matrigel differentiated cells compared to 2d culture plates. conclusion: overall, the results of this study suggest that huc-mscs can successfully differentiate toward dopaminergic neuron-like cells on matrigel, having great potential for the treatment of dopaminergic neuron-related diseases.
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کلیدواژه
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Differentiation ,Dopaminergic neurons ,Human umbilical cord mesenchymal stem cells ,In vitro
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آدرس
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ilam university of medical sciences, school of medicine, department of anatomy, Iran, iran university of medical sciences, ent and head and neck research center, five senses health institute, school of medicine, ent and head and neck department, Iran, mazandaran university of medical sciences, immunogenetic research center, school of medicine, department of anatomy & cell biology, Iran, mazandaran university of medical sciences, immunogenetic research center, school of medicine, department of anatomy & cell biology, Iran, ardabil university of medical sciences, school of medicine, department of anatomical sciences, research laboratory for embryology and stem cells, Iran, university of tehran, school of medicine, department of anatomy, Iran, ilam university of medical sciences, school of medicine, department of anatomy, Iran, qom university of medical sciences, school of medicine, department of anatomy, Iran, university of social welfare and rehabilitation sciences, school of rehabilitation sciences, department of basic sciences, Iran, golestan university of medical sciences, neuroscience research center, school of medicine, department of anatomy, Iran
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Authors
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