the repression of matrix metalloproteinases and cytokine secretion in glioblastoma by targeting k+ channel

Introduction: glioblastoma is an aggressive human brain malignancy with poorly understoodpathogenesis. voltage-gated potassium (kv) channels and matrix metalloproteinases (mmps)are highly expressed in malignant tumors and involved in the progression and metastasis ofglioblastoma. this study aimed to determine whether a voltage-dependent potassium channelblocker could modulate astrocytes as a cell involved in the immunopathogenesis of glioblastoma.methods: the cytotoxic effect of 4-aminopyridine (4-ap) at different doses in the cell modelof glioblastoma was measured by mtt assay. the elisa technique and gelatin zymographywere used to assess cytokine levels and mmp-9 after 4-ap treatment.results: cytotoxicity analysis data indicated that cell viability reduced by increasing 4-aplevel and cell growth decreased gradually by removing 4-ap from the cell medium. 4-apinhibits the secretion of il-6 and il-1 (p<0.05). mmp9 activity significantly inhibits withincreased 4-ap dose, compared to non-treated cells.conclusion: the reduction of cell viability, il-6 secretion, and mmp-9 activity in an in vitromodel of glioblastoma might be assumed 4-ap as an agent for chemoprevention of cancer.