نابودی سلول‌های سرطانی ملانوما توسط پلاسمای اتمسفری سرد در حضور نانوذرات طلا

ﻣﻘﺪﻣﻪ: ﻣﻼﻧﻮﻣﺎ، ﯾﮑﯽ از ﮐﺸﻨﺪه ﺗﺮﯾﻦ ﺳﺮﻃﺎن ﻫﺎی ﭘﻮﺳﺖ ﻣﯽ ﺑﺎﺷﺪ، زﯾﺮا اﺛﺮﺑﺨﺸﯽ درﻣﺎن ﻫﺎی راﯾﺞ ﭼﺸﻤﮕﯿﺮ ﻧﻤﯽ ﺑﺎﺷﺪ. ﭘﻼﺳﻤﺎی اﺗﻤﺴﻔﺮی ﺳﺮد راﻫﮑﺎری ﺟﺪﯾﺪ در درﻣﺎن ﺳﺮﻃﺎن ﻣﺤﺴﻮب ﻣﯽ ﺷﻮد. اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺑﺎ ﻫﺪف ﺑﺮرﺳﯽ اﺛﺮ ﻫﻢ اﻓﺰاﯾﯽ ﭘﻼﺳﻤﺎی ﺳﺮد در ﺣﻀﻮر ﻧﺎﻧﻮذرت ﻃﻼ ﺑﺮ رده ی ﺳﻠﻮﻟﯽ ﻣﻼﻧﻮﻣﺎ اﻧﺠﺎم ﺷﺪ. روش ﻫﺎ: در اﯾﻦ ﻣﻄﺎﻟﻌﻪ، از دﺳﺘﮕﺎه ﭘﻼﺳﻤﺎ ﺑﺎ ﮔﺎز ﻫﻠﯿﻮم در ﺣﻀﻮر ﻧﺎﻧﻮذرات ﻃﻼ ﺟﻬﺖ ﺗﯿﻤﺎر ﺳﻠﻮل ﻫﺎی ﻣﻼﻧﻮﻣﺎ اﺳﺘﻔﺎده ﺷﺪ. ﺑﺪﯾﻦ ﻣﻨﻈﻮر ﺑﻌﺪ از ﺳﻨﺘﺰ ﻧﺎﻧﻮذرات ﻃﻼ، ﺧﻮاص ﻓﯿﺰﯾﮑﻮﺷﯿﻤﯿﺎﯾﯽ آن ﻫﺎ ﺑﺮرﺳﯽ ﺷﺪ. ﺳﭙﺲ زﻣﺎن اﻧﮑﻮﺑﺎﺳﯿﻮن ﻧﺎﻧﻮذرات ﻃﻼ ﺗﻌﯿﯿﻦ و ﻏﻠﻈﺖ ﺑﻬﯿﻨﻪ ﭘﺲ از 24 ﺳﺎﻋﺖ اﻧﮑﻮﺑﺎﺳﯿﻮن ﻣﺸﺨﺺ ﮔﺮدﯾﺪ. در ﻧﻬﺎﯾﺖ ﺳﻠﻮل ﻫﺎ ﺑﺎ و ﺑﺪون ﺣﻀﻮر ﻧﺎﻧﻮذرات ﻃﻼ ﺗﺤﺖ ﺗﺎﺑﺶ ﭘﻼﺳﻤﺎی ﺳﺮد در زﻣﺎن ﻫﺎی ﻣﺨﺘﻠﻒ 60 ،30 و 90 ﺛﺎﻧﯿﻪ ﻗﺮار ﮔﺮﻓﺘﻨﺪ. درﺻﺪ ﺑﻘﺎی ﺳﻠﻮل ﻫﺎ، 48 ﺳﺎﻋﺖ ﺑﻌﺪ از درﻣﺎن ﺑﺎ ﭘﻼﺳﻤﺎی ﺳﺮد ﺑﺎ اﺳﺘﻔﺎده از آزﻣﻮن ﺑﻘﺎی ﺳﻠﻮﻟﯽ ارزﯾﺎﺑﯽ ﮔﺮدﯾﺪ. ﯾﺎﻓﺘﻪ ﻫﺎ: درﻣﺎن ﭘﻼﺳﻤﺎی ﺳﺮد، اﺛﺮ ﺳﻤﯿﺘﯽ واﺑﺴﺘﻪ ﺑﻪ زﻣﺎن اﯾﺠﺎد ﻣﯽ ﮐﻨﺪ. درﺻﺪ ﺑﻘﺎی ﺳﻠﻮل ﻫﺎی ﺗﯿﻤﺎر ﺷﺪه ﺑﺎ ﭘﻼﺳﻤﺎی ﺳﺮد در ﺣﻀﻮر ﻧﺎﻧﻮذرات ﻃﻼ ﺑﻪ ﻃﻮر ﻣﻌﻨﯽ داری ﻧﺴﺒﺖ ﺑﻪ ﺳﻠﻮل ﻫﺎی ﺗﯿﻤﺎر ﻧﺸﺪه ﮐﺎﻫﺶ ﯾﺎﻓﺖ. ﺑﯿﺸﺘﺮﯾﻦ ﮐﺎﻫﺶ درﺻﺪ ﺑﻘﺎ در ﮔﺮوه درﻣﺎﻧﯽ 90 ﺛﺎﻧﯿﻪ ﭘﻼﺳﻤﺎی ﺳﺮد و ﻏﻠﻈﺖ 20 mg/ml ﻧﺎﻧﻮذرات ﻃﻼ ﻣﺸﺎﻫﺪه ﮔﺮدﯾﺪ. ﻧﺘﯿﺠﻪ ﮔﯿﺮی: ﻣﻄﺎﻟﻌﻪ ی ﺣﺎﺿﺮ، ﺧﺎﺻﯿﺖ ﺿﺪﺳﺮﻃﺎﻧﯽ ﭘﻼﺳﻤﺎی اﺗﻤﺴﻔﺮی ﺳﺮد را در درﻣﺎن ﺳﻠﻮل ﻫﺎی ﻣﻼﻧﻮﻣﺎ ﺗﺄﯾﯿﺪ ﮐﺮد. ﻫﻤﭽﻨﯿﻦ ﻧﺸﺎن داد ﮐﻪ ﻧﺎﻧﻮذرات ﻃﻼ در درﻣﺎن ﺑﺎ ﭘﻼﺳﻤﺎ اﺛﺮ ﻫﻢ اﻓﺰاﯾﯽ اﯾﺠﺎد ﻣﯽ ﮐﻨﻨﺪ.

destruction of melanoma cells by cold atmospheric plasma in the presence of gold nanoparticles

background: melanoma is one of the deadliest skin cancers because the effectiveness of common treatments is not impressive. cold atmospheric plasma is considered a new solution in cancer treatment. this study was conducted with the aim of investigating the synergistic effect of cold plasma in the presence of gold nanoparticles on melanoma cell lines.methods: in this study, helium based cold plasma in the presence of gold nanoparticles were used to treat melanoma cells. in this respect, after the synthesis of gold nanoparticles, their physicochemical properties were investigated. then incubation time of gold nanoparticles was determined and the optimal concentration was determined after 24 hours of incubation using mtt test. finally, cells were exposed to cold plasma radiation with and without gold nanoparticles at different times of 30, 60 and 90 s. the survival percentage of cells was evaluated 48 hours after cold plasma treatment.findings: cold plasma treatment produces time-dependent toxic effects. the viability of cells treated with cold plasma in the presence of gold nanoparticles was significantly reduced compared to untreated cells. the greatest reduction in viability was observed in the 90 s cold plasma and 20 mg/ml concentration gold nanoparticle treatment groups.conclusion: this study approved anti-cancer effect of cold plasma on melanoma cells. in addition, it also showed that gold nanoparticles create a synergistic effect in plasma treatment.