گزارش یک مورد نادر استئوسارکوم اولیه‌ی دنده در یک مرد 70 ساله

مقدمه: تومورهای بدخیم اولیه‌ی دیواره‌ی قفسه‌ی صدری نادر است و حدود 5 درصد کل تومورهای دیواره‌ی قفسه‌ی سینه را تشکیل می‌دهد. استئوسارکوم اولیه‌ی دنده شیوع بسیار کمی دارد و بیشتر در جوانان دیده شده است.گزارش مورد: ما مرد 70 ساله‌ای را معرفی می‌کنیم که سابقه‌ی یک توده‌ی بسیار بزرگ دیواره‌ی قفسه‌ی صدری را از حدود 30 سال قبل می‌داد که به تدریج بزرگ‌تر شده بود. این توده تحت رزکشن و بازسازی قرار گرفت و در بررسی پاتولوژی، تشخیص well differentiated osteosarcoma گزارش شد.

A Rare Primary Rib Osteosarcoma in a 70yearold Man

<ul><li><div class=abs><strong>Background:</strong> Utilizing an intact RNA is essential while performing molecular genetics methods like microarray and quantitative <a href=http://en.wikipedia.org/wiki/Realtime_polymerase_chain_reaction>Realtime polymerase chain reaction </a>(RTPCR). Using RNA with poor quality may affect the results of downstream manipulations. Our aim in the current study was to compare and optimize various procedures for RNA extraction by using different kits and DNase treatment to find a standard method for RNA extraction from human frozen tissues.</div></li><li><div class=abs><strong>Methods</strong><strong>: </strong>Using Cinnagen RNX plus solution, Qiagen RNeasy Mini Kit and Qiagen RNeasy Plus Mini Kit, total RNA was extracted from human frozen tissues comprising stomach (#40), glioma (#5) and breast (#15). For genomic DNA elimination, extracted RNA was treated with DNase enzyme. After RNA extraction, its quantity and quality was assessed by spectrophotometry and gel electrophoresis. After cDNA synthesis, RTPCR technique by specific primers designed for <em>TBP</em> gene was performed and results were assessed by agarose gel electrophoresis.</div></li><li><strong>Finding: </strong>Extracted RNA by Cinnagen RNX plus solution did not show good results in RTPCR after DNase treatment. Extracted RNAs by Qiagen RNeasy Mini Kit and Qiagen RNeasy Plus Mini Kit were of high quality for RTPCR reaction because of using DNase treatment during the extraction procedure and an additional genomic DNA elimination column respectively.<strong></strong></li><li><strong>Conclusion: </strong>Combining Qiagen RNeasy Mini Kit, Qiazol solution and DNase treatment during the extraction procedure is the most suitable method for RNA extraction from human frozen tissues and the extracted RNA can be used with assurance in downstream molecular techniques.</li></ul>