migration of human wharton’s jelly stem cells seeded onto the chorioallantoic membrane into the chick liver

Background: developing chicken embryo culture techniques provides methods for manipulating chicken embryos. in this study, the cell-based approach investigated human wharton›s jelly stem cells (hwjscs) proliferation and migration into internal embryonic chick tissue.methods: wharton’s jelly cells were cultured on an acellular dermal matrix (adm) and seeded on the chorioallantoic membrane (cam) of a 4-day-old chicken embryo. using an rt-pcr assay with the cola-i human gene, molecular analysis was performed on chicken liver tissue after hatching to confirm the presence of these cells.results: the chicken liver tissue from wharton’s jelly stem cells/adm sample tested positive for the human cola-i gene. the cola-i gene sequence analysis shows this sequence belongs to the cola-i gene in humans. the cells cultured on cam can migrate and proliferate in the liver of a chicken embryo.conclusion: these findings suggest that human cells cultured on the cam can appear in the liver of chickens. however, further investigations are necessary to confirm this finding.