the effect of high-intensity interval training in the presence of l-carnitine on the expression of mct1, pgc-1, and cs in the liver of male wistar rats

Background: t liver oxidative metabolism disorder is dependent on several factors that lead to liver diseases like non-alcoholic fatty liver disease. our hypothesis was that the combination of high-intensity interval training (hiit) and l-carnitine affects the expression of citrate synthase (cs), pgc-1α, and monocarboxylate transporter 1 (mct1) in the liver of male wistar rats.methods: thirty-two male wistar rats were divided into four groups (n=8): control, l-carnitine (200 mg/kg/d, four weeks, ip), hiit, and hiit+l-carnitine (200 mg/kg/d, four weeks, ip). hiit training was performed for four weeks (five days a week); then, the animals were anesthetized, and their liver tissues were extracted for real-time pcr to measure mct1, pgc-1α, and cs. spss 22 software was used to analyze the data obtained in different groups, with p<0.05 considered statistically significant.results: mct1 expression significantly increased in the hiit and hiit+l-carnitine groups compared to the control group (p<0.001). pgc- 1α expression significantly increased in the hiit+l-carnitine group compared to the control and l-carnitine groups (p<0.001). also, pgc- 1α expression significantly increased in the hiit group compared to the control group (p<0.001). furthermore, cs expression significantly increased in all groups compared to the control group (p<0.001).conclusion: hiit exercise combined with l-carnitine supplementation increases the expression of mct1, pgc-1α, and cs in the liver. therefore, it seems that performing hiit exercises and taking l-carnitine supplements can prevent the consequences of fat accumulation in the liver.