The Relationship between the Expression of Small Heat-Shock Proteins and Suppression of OCT4B1

Background: oct4b1, a variant of oct4, is expressed in both cancer cells and tissues. this variant has a main role in the regulation of both apoptotic and stress (heat-shock proteins) pathways. the aim of this study was to investigate the effects of oct4b1 silencing on the expression of small heat-shock proteins (shsps)in three human tumor cell lines. methods:ags (gastric adenocarcinoma), 5637 (bladder tumor) and u-87mg (brain tumor) cell lines were transfected with specific oct4b1 sirna (test group) and scramble sirna (control group), using sirna and lipofectamine. real-time pcr array technique was applied and the fold changes were calculated using rt2 profiler pcr array data analysis version 3.5. results: it was revealed that hspb1, hspb6 and hspe1 were down-regulated in all three studied tumor cell lines and hspb2, hspb7 and hspb8 were down-regulated in two of three studied tumor cell lines (ags and 5637). it was also revealed that hspb3 was down-regulated in 5637 cell line and up-regulated in ags and u87mg cell lines. conclusion: according to the results, it may be concluded that there is a direct relationship between oct4b1 and shsps gene family expression. thus, suppression of oct4b1 may be considered in cancer therapy/research.