polymorphism of vascular endothelial growth factor gene−1154 g/a in women with recurrent miscarriage and ivf failure; a case–control study

Background: vascular endothelial growth factor a (vegfa) plays a key role in angiogenesis during human placenta formation and its abnormal expressions have been reported in placental tissues of women with recurrent miscarriage (rm). objective: the aim of this study was to evaluate the correlation of polymorphism of vegf1154 g/a gene in rm and in vitro fertilization (ivf) failures. methods: the peripheral blood samples of women with rm, ivf failures and healthy women with live born children, as control group were collected. dna samples were isolated and vegf 1154g/a polymorphism was evaluated by polymerase chain reaction and restriction fragment length polymorphism. single nucleotide polymorphism scanning was done using mnii restriction enzymes for 1154 g/a. results: the findings of this study showed that the vegf 1154 a/a and vegf 1154 g/a mutation frequencies in both rm and ivf failure groups were significantly higher than those in the control group (p=0.005). the homozygous aa mutant genotype frequency in the control group was 0, while in the rm and ivf failure groups it was 30% and 13.9%, respectively (p=0.005). moreover, the heterozygous ag genotype frequencies were higher in the rm (66.7%) and ivf failure (77.8%) compared to those in the control group (58.3%). conclusions: it was concluded that vegf 1154 a/a and vegf 1154 g/a polymorphisms were associated with both rm and ivf failures. however, their relation with ivf failures was more common than rm in a/g genotype, while in a/a, the rm was higher than ivf failure.