antibacterial and biological activity of polar and non-polar fractions of acroptilon repens (l.) dc against bap gene expression in acinetobacter baumannii biofilm

Background: acinetobacter baumannii is a major hospital drug-resistant pathogen. biofilm-related genes (bap)-specific proteins influence biofilm formation in a. baumannii. objectives: the present study examined the effect of the ethanolic extract, ethyl acetate, chloroform, and water fractions of acroptilon repens as a novel approach with antimicrobial and antibiofilm properties against a. baumannii. methods: this study used disk diffusion, well diffusion, and mic-mbc determination to screen the antibacterial activity of a. repens against a. baumannii. the antibiofilm properties of a. repens extract and its fractions were evaluated using a microtiter plate assay. the expression of the bap genes in sub-mbc-treated and non-treated a. baumannii was assessed using real-time pcr. results: the results indicated that the extract and fractions of a. repens exhibited significant antibacterial and antibiofilm properties. among the fractions, the chloroform and water fractions showed the highest antibiofilm effect on a. baumannii compared to the ethanolic extract and ethyl acetate fraction (p < 0.0001). the expression of the bap gene decreased after treatment with sub-mbc fractions of a. repens (p < 0.0001). conclusions: it can be concluded that a. repens extract and its fractions can be considered a new antibacterial and antibiofilm approach against a. baumannii.