assessing the expression levels of mir-1307-3p and mir-3613-5p in covid-19 patients

Background: micrornas (mirnas) regulate the immune response to infectious diseases. they exhibit alterations during the early stages of disease development, even before the pathogen is detectable.objectives: the objective of this study was to analyze the expression levels of mir-1307-3p and mir-3613-5p at the onset of covid-19, aiming to identify mirnas that could function as early predictive biomarkers.methods: this case-control study analyzed the expression levels of mir-1307-3p and mir-3613-5p at the onset of covid-19 to identify early predictive biomarkers. plasma mirnas from 26 covid-19 patients hospitalized in the icu ward were sequenced and compared to a healthy control group of 26 individuals. additionally, plasma biomarkers associated with the immune system were evaluated. total rna was extracted using an rnx solution and subjected to cdna synthesis with a specific loop sequence for each mirna. the samples were subsequently analyzed using relative real-time pcr.results: rt-qpcr analysis revealed a significant reduction in the detection rate of mir-1307-3p (p < 0.0001) in the patient group compared to the control group. conversely, no significant differences were observed in the levels of mir-3613-5p between the patient and control groups.conclusions: mir-1307-3p shows potential as a biomarker for the diagnosis of covid-19.